Fnames

bin/report.py

@@ -305,9 +305,7 @@ def gff_compare_plots(report, gffcompare_outdirs: Path, sample_ids):
 
     * **Totals**:
     Comparison of the number of stringtie-generated
-    transcripts, multiexonic transcripts and
+    transcripts, multiexonic transcripts and loci between reference and query.
-    loci (I'm not exactly sure what defines this class at the moment) between
-    reference
 
     * **Performance**:
     How accurate are the query transcript annotations with respect to the
@@ -411,6 +409,9 @@ def gff_compare_plots(report, gffcompare_outdirs: Path, sample_ids):
 
         tracking.drop(columns=['sample_id', 'Overlaps'], inplace=True)
         tracking = tracking[['Code', 'Description', 'Count', 'Percent']]
+        tracking = tracking.round({
+            'Percent': 2
+        })
 
         cols = [TableColumn(
             field=Ci, title=Ci, width=100) for Ci in tracking.columns]

main.nf

@@ -133,13 +133,17 @@ process split_bam{
     if (params["bundle_min_reads"] != false)
         """
         seqkit bam -j ${params.threads} -N ${params.bundle_min_reads} ${bam} -o  bam_bundles/
-            mv bam_bundles/* .
+        mv bam_bundles/* .
-        for f in *:*; do mv -v "\$f" \$(echo "\$f" | tr ':' '-'); done
+        let i=1
+        for b in *.bam; do
+          newname="${sample_id}_batch_\${i}.bam"
+          mv \$b \$newname
+          ((i++))
+        done
         """
     else
         """
-        mkdir -p ./${sample_id}_bam_bundles
+        ln -s ${bam} ${sample_id}_batch_1.bam
-        ln -s ${bam} ${sample_id}_bam_bundles-000000000-ALL-0-1_bundle.bam
         """
 }
 
@@ -161,17 +165,12 @@ process assemble_transcripts{
     output:
         tuple val(sample_id), path('*.gff'), emit: gff_bundles
     script:
-        def out_filename = bam.name.replaceFirst(~/\.[^\.]+$/, '') + "_${sample_id}.gff"
         def G_FLAG = ref_annotation.name.startsWith('OPTIONAL_FILE') ? '' : "-G ${ref_annotation}"
-        // Convert batch name to stringtie prefix to prevent clashing attribute names
+        def prefix =  bam.name.split(/\./)[0]
-        // eg "0000000123_cluster..." to 123
+
-        def prefix =  StringUtils.stripStart(bam.name.split('_')[0],"0")
-        if (!prefix){
-            prefix = "0"
-        }
     """
-    stringtie --rf ${G_FLAG} -L -v -A gene_abund.tab -p ${params.threads} ${params.stringtie_opts} -o  ${out_filename} \
+    stringtie --rf ${G_FLAG} -L -v -p ${params.threads} ${params.stringtie_opts} \
-        -l $prefix ${bam} 2>/dev/null
+    -o  ${prefix}.gff -l ${prefix} ${bam} 2>/dev/null
      """
 }