diff --git a/CHANGELOG.md b/CHANGELOG.md
index aa6090b..ba25e70 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -9,6 +9,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### Fixed
 - Incorrect strand assignment when mapping cDNA reads is fixed by using minimap2 `-ub` instead of `-uf`.
+- Remove mention of analysis_group and type from `README.md` as they are not relevant for this workflow.
 
 ## [v2.0.1]
 
diff --git a/README.md b/README.md
index be7d1dd..73fd42f 100644
--- a/README.md
+++ b/README.md
@@ -200,10 +200,6 @@ sample aliases to be mapped to barcodes in multiplexed runs and is required for
   `barcode02`, and the values must be unique.
 + `alias` is the user-facing sample name, must be unique, must not begin
   with the word `barcode` and may contain only letters, numbers, `.`, `_` or `-`.
-+ If a `type` column is present, it must use one of:
-  `test_sample`, `positive_control`, `negative_control`, or
-  `no_template_control`.
-+ If an `analysis_group` column is present, every row must have a value.
 + For `--de_analysis`, the sheet must also contain the primary condition
   column (`condition` by default, overridable with `--condition_column`),
   plus any columns named in `--covariates`.
diff --git a/docs/06_pipeline_overview.md b/docs/06_pipeline_overview.md
index 2abc8d2..faeef1c 100644
--- a/docs/06_pipeline_overview.md
+++ b/docs/06_pipeline_overview.md
@@ -39,10 +39,6 @@ sample aliases to be mapped to barcodes in multiplexed runs and is required for
   `barcode02`, and the values must be unique.
 + `alias` is the user-facing sample name, must be unique, must not begin
   with the word `barcode` and may contain only letters, numbers, `.`, `_` or `-`.
-+ If a `type` column is present, it must use one of:
-  `test_sample`, `positive_control`, `negative_control`, or
-  `no_template_control`.
-+ If an `analysis_group` column is present, every row must have a value.
 + For `--de_analysis`, the sheet must also contain the primary condition
   column (`condition` by default, overridable with `--condition_column`),
   plus any columns named in `--covariates`.