tag v0.4.2
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@ -4,8 +4,11 @@ All notable changes to this project will be documented in this file.
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The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.1.0/),
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and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).
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## [unreleased]
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## [v0.4.2]
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### Changed
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- Sample sheet must include a `control` type to indicate which samples are the reference for the differential expression pipeline.
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### Removed
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- Default local executor CPU and RAM limits.
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## [v0.4.1]
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### Changed
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@ -2,6 +2,7 @@
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import codecs
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import csv
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import os
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import re
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import sys
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from .util import get_named_logger, wf_parser # noqa: ABS101
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@ -79,6 +80,19 @@ def main(args):
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sys.stdout.write(f"Parsing error: {e}")
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sys.exit()
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# check barcodes are correct format
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for barcode in barcodes:
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if not re.match(r'^barcode\d\d+$', barcode):
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sys.stdout.write("values in 'barcode' column are incorrect format")
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sys.exit()
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# check barcodes are all the same length
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first_length = len(barcodes[0])
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for barcode in barcodes[1:]:
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if len(barcode) != first_length:
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sys.stdout.write("values in 'barcode' column are different lengths")
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sys.exit()
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# check barcode and alias values are unique
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if len(barcodes) > len(set(barcodes)):
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sys.stdout.write("values in 'barcode' column not unique")
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@ -110,15 +110,9 @@ manifest {
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description = 'Transcriptome analysis including gene fusions, differential expression as well as assembly and annotation of cDNA and direct RNA sequencing data.'
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mainScript = 'main.nf'
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nextflowVersion = '>=23.04.2'
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version = 'v0.4.1'
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version = 'v0.4.2'
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}
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executor {
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$local {
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cpus = 4
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memory = "8 GB"
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}
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}
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epi2melabs {
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tags = "isoforms, transcriptomics"
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