{ "files": { "workflow-report": { "filepath": "wf-transcriptomes-report.html", "title": "Workflow report", "description": "HTML report summarising transcript discovery, quantification, optional SQANTI3 classification, and optional differential analysis results.", "mime-type": "text/html", "optional": false, "type": "aggregated" }, "read-stats-per-file": { "filepath": "ingress_results/{{ alias }}/fastcat_stats/per-file-stats.tsv", "title": "Per-file read stats", "description": "Read statistics for each input FASTQ file in a sample, when FASTQ read stats are available.", "mime-type": "text/tab-separated-values", "optional": true, "type": "per-sample" }, "read-stats-per-read": { "filepath": "ingress_results/{{ alias }}/fastcat_stats/per-read-stats.tsv.gz", "title": "Per-read stats", "description": "Read statistics for individual reads in a sample, when this output is enabled.", "mime-type": "application/gzip", "optional": true, "type": "per-sample" }, "sample-fastq": { "filepath": "ingress_results/{{ alias }}/seqs.fastq.gz", "title": "Ingress reads", "description": "Reads prepared from the input data for downstream analysis.", "mime-type": "application/gzip", "optional": true, "type": "per-sample" }, "sample-metadata": { "filepath": "ingress_results/{{ alias }}/metamap.json", "title": "Ingress metadata", "description": "Per-sample metadata used by the workflow.", "mime-type": "application/json", "optional": true, "type": "per-sample" }, "sample-bam": { "filepath": "cohort/alignments/{{ alias }}.aligned.sorted.bam", "title": "Aligned BAM", "description": "Genome-aligned BAM used for bambu, optional SQANTI3 QC, and IGV.", "mime-type": "application/gzip", "optional": false, "type": "per-sample" }, "sample-bai": { "filepath": "cohort/alignments/{{ alias }}.aligned.sorted.bam.bai", "title": "Aligned BAM index", "description": "Index for the aligned BAM.", "mime-type": "application/octet-stream", "optional": false, "type": "per-sample" }, "sample-flagstat": { "filepath": "cohort/alignments/{{ alias }}.flagstat.txt", "title": "Alignment summary", "description": "samtools flagstat output for the aligned BAM.", "mime-type": "text/plain", "optional": false, "type": "per-sample" }, "annotation-reference-summary": { "filepath": "cohort/reference/annotation_reference_summary.json", "title": "Reference and annotation preparation summary", "description": "Summary of reference and annotation preparation, including seqname overlap, build/provider hints, and excluded unstranded annotation counts.", "mime-type": "application/json", "optional": false, "type": "aggregated" }, "unstranded-annotation": { "filepath": "cohort/reference/unstranded_annotation.gtf", "title": "Excluded unstranded annotation records", "description": "Full set of annotation records excluded because their strand was not '+' or '-'. Present only when unstranded records are found.", "mime-type": "text/plain", "optional": true, "type": "aggregated" }, "cohort-gtf": { "filepath": "cohort/transcripts.gtf", "title": "Cohort transcriptome GTF", "description": "Joint bambu transcript model used as the primary cohort transcriptome.", "mime-type": "text/plain", "optional": false, "type": "aggregated" }, "cohort-fasta": { "filepath": "cohort/cohort.transcriptome.fa", "title": "Cohort transcriptome FASTA", "description": "Transcript sequences derived from the joint cohort GTF.", "mime-type": "text/plain", "optional": false, "type": "aggregated" }, "cohort-transcript-counts": { "filepath": "cohort/transcript_counts.tsv", "title": "Cohort transcript counts", "description": "Transcript-level count matrix produced by bambu.", "mime-type": "text/tab-separated-values", "optional": false, "type": "aggregated" }, "cohort-gene-counts": { "filepath": "cohort/gene_counts.tsv", "title": "Cohort gene counts", "description": "Gene-level count matrix derived from bambu output.", "mime-type": "text/tab-separated-values", "optional": false, "type": "aggregated" }, "cohort-transcript-metadata": { "filepath": "cohort/transcript_metadata.tsv", "title": "Cohort transcript metadata", "description": "Transcript annotations and bambu transcript classes for the cohort model.", "mime-type": "text/tab-separated-values", "optional": false, "type": "aggregated" }, "cohort-sqanti-summary": { "filepath": "cohort/sqanti_cohort/classification_summary.tsv", "title": "Cohort SQANTI3 summary", "description": "SQANTI3 classification summary for the cohort transcriptome when SQANTI3 QC is enabled.", "mime-type": "text/tab-separated-values", "optional": true, "type": "aggregated" }, "sample-gtf": { "filepath": "samples/{{ alias }}/transcripts.gtf", "title": "Per-sample transcriptome GTF", "description": "Independent bambu transcript model for an individual sample.", "mime-type": "text/plain", "optional": false, "type": "per-sample" }, "sample-fasta": { "filepath": "samples/{{ alias }}/{{ alias }}.transcriptome.fa", "title": "Per-sample transcriptome FASTA", "description": "Transcript sequences derived from the per-sample GTF.", "mime-type": "text/plain", "optional": false, "type": "per-sample" }, "sample-transcript-counts": { "filepath": "samples/{{ alias }}/transcript_counts.tsv", "title": "Per-sample transcript counts", "description": "Transcript-level abundance estimates for the per-sample bambu model.", "mime-type": "text/tab-separated-values", "optional": false, "type": "per-sample" }, "sample-gene-counts": { "filepath": "samples/{{ alias }}/gene_counts.tsv", "title": "Per-sample gene counts", "description": "Gene-level abundance estimates for the per-sample bambu model.", "mime-type": "text/tab-separated-values", "optional": false, "type": "per-sample" }, "sample-transcript-metadata": { "filepath": "samples/{{ alias }}/transcript_metadata.tsv", "title": "Per-sample transcript metadata", "description": "Transcript annotations and bambu transcript classes for the per-sample model.", "mime-type": "text/tab-separated-values", "optional": false, "type": "per-sample" }, "sample-sqanti-summary": { "filepath": "samples/{{ alias }}/{{ alias }}_sqanti/classification_summary.tsv", "title": "Per-sample SQANTI3 summary", "description": "SQANTI3 classification summary for the per-sample transcriptome when SQANTI3 QC is enabled.", "mime-type": "text/tab-separated-values", "optional": true, "type": "per-sample" }, "dge-results": { "filepath": "de_analysis/{{ contrast }}/results_dge.tsv", "title": "Differential gene expression results", "description": "DESeq2 gene-level differential expression results for one contrast.", "mime-type": "text/tab-separated-values", "optional": true, "type": "aggregated" }, "dge-report": { "filepath": "de_analysis/{{ contrast }}/results_dge.pdf", "title": "Differential gene expression plots", "description": "PDF plots generated during DESeq2 analysis for one contrast.", "mime-type": "application/pdf", "optional": true, "type": "aggregated" }, "dtu-transcript-results": { "filepath": "de_analysis/{{ contrast }}/results_dtu_transcript.tsv", "title": "Differential transcript usage results", "description": "Transcript-level DTU results for one contrast.", "mime-type": "text/tab-separated-values", "optional": true, "type": "aggregated" }, "dtu-gene-results": { "filepath": "de_analysis/{{ contrast }}/results_dtu_gene.tsv", "title": "Differential transcript usage gene summary", "description": "Gene-level DTU summary for one contrast.", "mime-type": "text/tab-separated-values", "optional": true, "type": "aggregated" }, "dexseq-results": { "filepath": "de_analysis/{{ contrast }}/results_dexseq.tsv", "title": "DEXSeq results", "description": "Full DEXSeq result table for one contrast.", "mime-type": "text/tab-separated-values", "optional": true, "type": "aggregated" }, "dtu-report": { "filepath": "de_analysis/{{ contrast }}/results_dtu.pdf", "title": "Differential transcript usage plots", "description": "PDF plots generated during DEXSeq analysis for one contrast.", "mime-type": "application/pdf", "optional": true, "type": "aggregated" }, "igv-config": { "filepath": "igv.json", "title": "IGV configuration", "description": "JSON configuration for viewing the aligned BAMs in IGV.", "mime-type": "text/json", "optional": true, "type": "aggregated" }, "reference-index": { "filepath": "igv_reference/{{ ref_genome_file }}.fai", "title": "Reference FASTA index", "description": "FAI index for the reference genome published for IGV.", "mime-type": "text/tab-separated-values", "optional": true, "type": "aggregated" }, "reference-gzi-index": { "filepath": "igv_reference/{{ ref_genome_file }}.gzi", "title": "Reference GZI index", "description": "GZI index for a compressed reference genome published for IGV.", "mime-type": "application/octet-stream", "optional": true, "type": "aggregated" } } }