ONT wf-transcriptomes v2.0.2 for DAU validation
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Workflow template

This repository contains a nextflow workflow template and associated container build.

Quickstart

# build the container
CONTAINER_TAG=template-workflow
docker build \
    -t ${CONTAINER_TAG} -f Dockerfile \
    --build-arg BASEIMAGE=epi2melabs/base-workflow-image:latest \
    .

The BASEIMAGE argument here can be changed to use an alternative image.

The template includes a simple workflow that outputs a file with the lengths of sequences contained in a .fastq.gz file.

# run the pipeline with the test data
OUTPUT=template-workflow
nextflow run workflow.nf \
    -w ${OUTPUT}/workspace \
    -profile standard \
    --reads test_data/reads.fq.gz \
    --out_dir ${OUTPUT}

The output of the pipeline will be found in ./template-workflow for the above example. This directory contains the nextflow working directories alongside the two primary outputs of the pipeline.