wf-transcriptomes-v202/docs/07_outputs.md
2024-02-02 10:38:50 +00:00

4.4 KiB

Output files may be aggregated including information for all samples or provided per sample. Per-sample files will be prefixed with respective aliases and represented below as {{ alias }}.

Title File path Description Per sample or aggregated
workflow report wf-transcriptomes-report.html a HTML report document detailing the primary findings of the workflow aggregated
Per file read stats fastq_ingress_results/reads/fastcat_stats/per-file-stats.tsv A TSV with per file read stats, including all samples. aggregated
Read stats fastq_ingress_results/reads/fastcat_stats/per-read-stats.tsv A TSV with per read stats, including all samples. aggregated
Run ID's fastq_ingress_results/reads/fastcat_stats/run_ids List of run IDs present in reads. aggregated
Meta map json fastq_ingress_results/reads/metamap.json Metadata used in workflow presented in a JSON. aggregated
Concatenated sequence data fastq_ingress_results/reads/{{ alias }}.fastq.gz Per sample reads concatenated in to one FASTQ file. per-sample
Assembled transcriptome {{ alias }}_transcriptome.fas Per sample assembled transcriptome. per-sample
Annotated assembled transcriptome {{ alias }}_merged_transcriptome.fas Per sample annotated assembled transcriptome. per-sample
Alignment summary statistics {{ alias }}_read_aln_stats.tsv Per sample alignment summary statistics. per-sample
GFF compare results. {{ alias }}_gffcompare All GFF compare output files. per-sample
Differential gene expression results /de_analysis/results_dge.tsv This is a gene-level result file that describes genes and their probability of showing differential expression between experimental conditions. aggregated
Differential gene expression report /de_analysis/results_dge.pdf Summary report of differential gene expression analysis as a PDF. aggregated
Differential transcript usage gene TSV /de_analysis/results_dtu_gene.tsv This is a gene-level result file from DEXSeq that lists annotated genes and their probabilities of differential expression. aggregated
Differential transcript usage report /de_analysis/results_dtu.pdf Summary report of differential transcript usage results as a PDF. aggregated
Differential transcript usage TSV /de_analysis/results_dtu_transcript.tsv This is a transcript-level result file from DEXSeq that lists annotated genes and their probabilities of differential expression. aggregated
Differential transcript usage stageR TSV /de_analysis/results_dtu_stageR.tsv This is the output from StageR and it shows both gene and transcript probabilities of differential expression aggregated
Differential transcript usage DEXSeq TSV /de_analysis/results_dexseq.tsv The complete output from the DEXSeq-analysis, shows both gene and transcript probabilities of differential expression. aggregated
Gene counts /de_analysis/all_gene_counts.tsv Raw gene counts created by the Salmon tool, before filtering. aggregated
Gene counts per million /de_analysis/cpm_gene_counts.tsv This file shows counts per million (CPM) of the raw gene counts to facilitate comparisons across samples. aggregated
Transcript counts /de_analysis/unfiltered_transcript_counts_with_genes.tsv Raw transcript counts created by the Salmon tool, before filtering. Includes reference to the associated gene ID. aggregated
Transcript per million counts /de_analysis/unfiltered_tpm_transcript_counts.tsv This file shows transcripts per million (TPM) of the raw counts to facilitate comparisons across samples. aggregated
Transcript counts filtered /de_analysis/filtered_transcript_counts_with_genes.tsv Filtered transcript counts, used for differential transcript usage analysis. Includes a reference to the associated gene ID. aggregated
Final non redundant transcriptome /de_analysis/final_non_redundant_transcriptome.fasta Transcripts that were used for differential expression analysis including novel transcripts with the identifiers used for DE analysis. aggregated
Fusion transcript sequences /jaffal_output_{{ alias }}/jaffa_results.fasta Fusion transcript sequences output by Jaffa. per-sample
Fusion transcript sequence summary file /jaffal_output_{{ alias }}/jaffa_results.csv Fusion transcript sequences summary file output by Jaffa. per-sample